Other

Part:BBa_J70504:Design

Designed by: Tom Knight   Group: Knight Lab   (2009-10-03)


Mesoplasma florum origin of replication


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 928
    Illegal EcoRI site found at 1609
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 928
    Illegal EcoRI site found at 1609
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 928
    Illegal EcoRI site found at 1609
    Illegal BglII site found at 466
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 928
    Illegal EcoRI site found at 1609
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 928
    Illegal EcoRI site found at 1609
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR from genomic DNA


Source

Needs mutation of EcoRI sites and GATC sites.

References